Journal: bioRxiv

Article Title: NDNF+ interneurons have a privileged role in regulating cortical excitability

doi: 10.1101/2025.11.27.690945

Figure Lengend Snippet: ( A ) mCherry expression in NDNF+ neurons within the stratum lacunosum moleculare (sl-m) of the hippocampus from mice injected with either intrahippocampal saline (control) or intrahippocampal kainate (epileptic). DG = dentate gyrus. The number of mCherry-positive cells per area was unchanged in epileptic animals (the unpaired mean difference between Saline and Kainate is 26,3% [95%CI, 7.8, 50.5], p=0.072 two-sided permutation t-test, and p= 0.06 Student’s t-test, Saline n = 8 slices /3 mice; Kainate n= 20 slices /3 mice). ( B ) Experimental setup. ( C ) Representative LFP traces from hippocampal depth electrodes from a mouse expressing hM3Dq in NDNF+ neurons before and after intraperitoneal (i.p) injection of either DMSO (0.1% in saline) control or DCZ (10 μg/kg).

Article Snippet: In the meantime, an LFP recording electrode (borosilicate glass pipettes 1.5 mm OD, 0.86 mm ID Warner Instruments, G150F-4 with a resistance of 2-3 MΩ) filled with saline was positioned at the centre of the craniotomy above the pilocarpine injection site and lowered to 0.3 mm below the pia, and a bare ended optic fibre (ø: 200 μm, NA: 0.22, Multimode 190–1200 nm, Thorlabs Inc., UK) was positioned 1.5-2 mm above the pia ( Suppl.

Techniques: Expressing, Injection, Saline, Control

Journal: Optics Express

Article Title: Side-view holographic endomicroscopy via a custom-terminated multimode fibre

doi: 10.1364/oe.426235

Figure Lengend Snippet: Fig. 2. Optical setup for both straight- and side-view imaging probes. The calibration units are used to acquire the transmission matrix of each probe prior imaging. Following the calibration, a series of holograms is applied to a digital micromirror device, shaping the incoming laser beam. The modified wavefronts are coupled into the proximal fibre facet, which translates into focal points scanned across the distal end of the probe. Through the same fibre facet, the fluorescence signals emitted by a distal object are collected and detected by a photomultiplier tube on the proximal side, allowing reconstruction of an image. Legend: MMF, multimode fibre; DMD, digital micromirror device; ID, iris diaphragm; L, lens; HWP, half-wave plate; QWP, quarter-wave plate; BS, non-polarizing beamsplitter plate; PBS, polarizing beamsplitter cube; MO, microscope objective; Cam, camera; S, shutter; OI, optical isolator; M, mirror; DM, dichroic mirror; F, filter; PMT, photomultiplier tube detector. The detailed list of components can be found in the Appendix.

Article Snippet: Appendix The equipment and components used in this work are detailed as follows (refer to Fig. 2): Laser – Coherent Sapphire SF 488; OI – free-space optical isolator: Thorlabs IO-3-488-HP; HWP1, HWP2, HWP3, HWP4 – half-wave plate: Edmund multiple order M-17.5-D-.500-B-488; QWP1, QWP2, QWP3 – quarter-wave plate: Thorlabs WPMH10M-488; PBS1, PBS2 – polarizing beamsplitter cube: Thorlabs CCM1-PBS251/M; BS1, BS2 – beamsplitter plate: Thorlabs BSW10; DM – broadband dielectric mirror: Thorlabs BB1-E02, S – shutter: Thorlabs SHB05T; lens – L1, L6, L9: Thorlabs C240TMD-A, L2, L10: Thorlabs AC254-300-A-ML, L3: AC254250-A-ML, L4: Thorlabs AC254-040-A-ML, L5: Thorlabs AC254-125-A-ML, L7, L8: Thorlabs AC254-200-A-ML, L11: Thorlabs AC254-100-A-ML; DMD – digital micromirror device: Vialux V-7001; MMF – multimode fibre: CeramOptec Optran WFGe100/110/125 0.37; MO1, MO2 – microscope objective: Olympus 20X X-Apo 0.80NA/0.6WD; Cam1, Cam2 – camera: Basler acA640-750um; DM – widefield microscopy dichroic mirror: Chroma Technology T495lpxru; F – long pass filter: Thorlabs FEL0550; PMT – GaAsP amplified photomultiplier tube: Thorlabs PMT2101/M; photodiode – Thorlabs PDA36A2 (not depicted in Fig. 2; it is placed in the position of Cam1 or Cam2, for straight-view imaging and side-view imaging, respectively).

Techniques: Imaging, Transmission Assay, Modification, Fluorescence, Microscopy